Nucleic acid extraction and reverse transcription
Patient total nucleic acids were extracted from serum and whole blood
specimens utilizing the commercial nucleic acid extraction Kit
(EasyPure® Viral DNA/ RNA Kit ER201-01). The reverse transcription of
nucleic acids was performed employing the Kit (HiScript III 1st Strand
cDNA Synthesis Kit). All operations and procedures are performed in
accordance with the commercial kit’s instruction manual.