Figure 3. Methylation signature of land and water form ofRiccia fluitans
A. The histogram tracks (A-D) depict the logarithmic mean of expression patterns from the Illumina (A,B) and Nanopore (C,D) sequencing of the frequency water (A,C) and land (B,D) forms ofRiccia fluitans . The heatmap (E) represents the methylation levels of the transcript, with red indicating one methylation, orange indicating two methylations, yellow indicating three methylations, green indicating four, and blue indicating five methylations. The innermost track (F) presents the correlation links between experimental groups, where purple links depict intersected transcripts with methylations.
B. The Venn diagram depicts the number and percentage of unique methylations in terrestrial (orange), aquatic (blue), and common to both sets (dark orange) Riccia fluitans .
C. A logo diagram depicts the probability of a nucleotide appearing in the first five positions of the significant methylation motif in both the water (upper diagram) and land (lower diagram) forms. The larger the letter representing the nucleotide, the higher the probability of its appearance.
D. Upset plot of GO annotations for genes indicated high methylation probability in water environment. High bars describe the number of genes engaged in common GO terms. The dots and lines merge GO terms with common genes.
E. Upset plot of GO annotations for genes indicated high methylation probability in land environment. High bars describe the number of genes engaged in common GO terms. The dots and lines merge GO terms with common genes.