Figure 4. Polyadenylation signals detected by direct RNA
sequencingÂ
A. Volcano plot depicting log2 Fold Change (log2FC) for genes
with significant poly (A) tail. The x-axis displays the log2FC values
for each gene, while the y-axis shows the negative log-adjusted p-value
(p-adjusted). The horizontal dashed line represents the negative
logarithmic p-adjusted cutoff value (0.05), and the vertical line equals
the value of 0 log2FC. Red points indicate statistically significant
genes, while gray points represent non-significant genes.
B. Boxplot comparing the distribution of poly(A) tail lengths
in the CL.22730.2 transcript across study groups, water (blue) and land
(orange). The boxplot depicts the median, first and third quartiles
(lower and upper hinges), largest and smallest value (upper and lower
whisker).
C. Poly(A) tail length profiling of Riccia fluitans mRNA
depending on the living environment. A density distribution plot is
shown for the mRNA of all transcripts detected in Riccia fluitanscells in the aquatic environment (blue) and in the terrestrial
environment (orange). The vertical dashed lines represent the median
poly(A) tail length (in nucleotides).
D. Scatter plot of poly(A) tail length for differentRiccia fluitans clones, representing all transcripts from poly(A)
tail length profiling. Each point represents the length of poly(A) from
a clone grown in either an aquatic (blue) or terrestrial (land)
environment.