MATERİALS AND METHODS
This study was conducted with the approval of the Istanbul Aydin University Non-Interventional Clinical Research Ethics Committee with decision number 2023/16. Study groups were formed based on volunteerism, and individuals aged between 18-60 years without any chronic diseases and immune deficiencies were selected for obese and control groups. For obese patients, individuals with BMI ≥30 and for healthy groups, individuals with BMI greater than 18.5 and less than 25 were selected. 10 ml of fasting blood was taken from 48 patients and 42 healthy individuals who met these criteria. The collected blood samples were centrifuged to separate the sera and stored at -20°C until the study was conducted.
The seropositivity of Ad-37 and Ad-5 in the collected study samples was investigated using ELISA test kits, which can demonstrate antigen-antibody reactions through enzymes. The serum samples of obese and healthy individuals were tested according to the instructions of the test kits. Optical density measurements at a wavelength of 450 nm were made using the Biotek-Epoch brand ELISA microplate reader.
The obtained results were interpreted as positive or negative by taking the average of the standard samples included in the ELISA kits and accepting it as the cutoff value. Normality analyzes were examined to analyze the data, and it was observed that the data were not normally distributed. Independent two-group Mann-Whitney U test, a non-parametric analysis, was used to determine the statistical significance of the results and the relationship between them, and a significance level of p <0.05 was accepted.