2.6 Statistical analysis
SNP alleles and genotypes were described in terms of absolute number and
percentage. Hardy-Weinberg equilibrium (HWE) analysis was performed
using Pearson’s chi-square test. A web-based platform, SHEsis, was used
to perform LD and haplotype analyses
(http://analysis.bio-x.cn/myAnalysis.php). Risk assessment, odds
ratio (OR) and its 95% confidence interval (CI), was estimated using
age- and gender-adjusted multinomial logistic regression analysis, which
was performed under five genetic models; allele, recessive, dominant,
over-dominant, and co-dominant. Median and IQR were used to express
continuous variables because they did not follow a normal distribution
(Shapiro-Wilk and Kolmogorov–Smirnov tests), and Mann-Whitney U test
was used to assess significant differences. The discriminatory
performance of serum IFIH1 levels between patients and HC were evaluated
using receiver-operating characteristic (ROC) curve analysis, which was
presented in terms of area under the curve (AUC), 95% confidence
interval, cut-off point (adjusted with Youden index; YI), sensitivity,
and specificity. Correlation coefficient (rs) was
determined using Spearman’s rank-order correlation analysis. The level
of significance was set at a probability (p ) of less than 0.05,
which was corrected (pc ) for multiple comparisons with the
Bonferroni correction method or the false discovery rate method.
Statistical analysis was performed using IBM SPSS Statistics 25.0
(Armonk, NY: IBM Corp.) and graphs were generated using GraphPad Prism
version 9.2.0 (San Diego, CA, USA).